human recombinant fibroblast growth factor 10 (fgf10 (PeproTech)
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human recombinant fibroblast growth factor 10 (fgf10
Human Recombinant Fibroblast Growth Factor 10 (Fgf10, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+fibroblast+growth+factor+10+(fgf10/fgf10+growth+factor/pm39627437-179-50-59
Average 90 stars, based on 1 article reviews
Human Recombinant Fibroblast Growth Factor 10 (Fgf10, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+fibroblast+growth+factor+10+(fgf10/fgf10+growth+factor/pm39627437-179-50-59
Average 90 stars, based on 1 article reviews
human recombinant fibroblast growth factor 10 (fgf10 - by Bioz Stars,
2026-09
90/100 stars
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other:Article Title: Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays. Article Snippet: Isolated cells were placed into 12-well plates (Corning, #3513) coated with 150 μl growth factor reduced matrigel (Corning, Wiesbaden, Germany) and cultured with advanced DMEM/F12 supplemented with hydroxyethylpiperazineethanesulfonic acid (HEPES) (10mmol/L Thermo Fisher, Waltham, MA, USA), Wnt family member 3 A (WNT3A)-conditioned medium (30% v/v), R-spondin-1 (RSPO1)-conditioned medium (10% v/v); Article Title: Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Ki67 Thermo Scientific RM-9106-S; RRID:AB_2341197 Anti-E-cadherin BD Bioscience 610181; RRID:AB_397580 Anti-PCNA Santa Cruz sc-56; RRID:AB_628110 Anti-K19 Abcam ab76539; RRID:AB_1523469 Anti-ALB Sigma A6684; RRID:AB_258309 Anti-MRP2 Abcam ab187644; RRID:AB_3665246 Anti-LYZ Abcam ab74666; RRID:AB_1310422 Anti-VIL-1 Abcam ab201989; RRID:AB_3665247 Anti-SOX9 LSbioscciences LS-C148618; RRID:AB_11130372 Anti-mouse Alexa 488 ThermoFisher A-11029; RRID:AB_2534088 Anti-rabbit Alexa 488 ThermoFisher A-11034; RRID:AB_2576217 Anti-mouse Alexa 568 ThermoFisher A-11004; RRID:AB_2534072 Anti-rabbit Alexa 568 ThermoFisher A-11036; RRID:AB_10563566 Chemicals, peptides, and recombinant proteins HepatiCultTM Organoid Kit (Human) Stem Cell Technologies Catalog # 100-0386 Human HepatiCultTM Organoid Growth supplement Stem Cell Technologies Catalog # 100-0389 HepatiCultTM Organoid Differentiation supplement Stem Cell Technologies Catalog # 100-0388 Human IntestiCultTM Organoid Growth medium Stem Cell Technologies Catalog # 06010 Human IntestiCultTM Differentiation Medium Stem Cell Technologies Catalog # 100-0214 Basal medium (Advanced DMEM/F12 (AD)) Thermo Fisher Scientific Catalog # 12634028 GlutaMax Gibco Catalog # 35050061 HEPES Gibco Catalog # 15630-056 Penicillin-streptomycin Gibco Catalog # 15140-122 N2 Fisher Scientific Catalog # 17-502-001 B27 Thermo Scientific Catalog # 17504044 N-Acetylcysteine Sigma-Aldrich Catalog # A7250 RSPO1 conditioned media Home made NA Wnt3a conditioned media Home made NA Recombinant human (Leu15)-gastrin I Tebu-Bio Catalog # AS-64149 EGF Invitrogen Catalog # Recombinant:Article Title: Chemically Defined Organoid Culture System for Cholangiocyte Differentiation. Article Snippet: .. The EM was consisted of Advanced DMEM/F12 medium (Gibco) supplemented with 1% (v/v) P/S, 10 × 10−3 m HEPES (Gibco), 1% (v/v) GlutaMax (Gibco), 10% (v/v) Rspondin-1 conditioned medium (the Rspon1-Fcexpressing cell line was a kind gift from Calvin J. Kuo), 2% (v/v) B27 supplement without vitamin A (Invitrogen, Carlsbad, CA), 1% (v/v) N2 supplement (Invitrogen), 1.25 × 10−3 m N-acetylcysteine (NAC; Sigma-Aldrich, St. Louis, MO), 10× 10−3 m nicotinamide (Sigma-Aldrich), 10× 10−9 m recombinant human (Leu15)-gastrin I (GAS; Tocris Bioscience, Bristol, UK), 50 ng mL−1 epidermal growth factor (EGF; Peprotech, Rocky Hill, NJ), 25 ng mL−1 hepatocyte growth factor (HGF; Peprotech), 100 ng mL−1 Article Title: Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor Article Snippet: .. For pancreatic ductal organoids (PDOs) culture, EM composition for PDO expansion was prepared as previously described : Basal medium (Advanced DMEM/F12 (AD, Gibco), containing 1% (v/v) GlutaMax (Gibco), HEPES (10 mM, Gibco) and 1% (v/v) penicillin-streptomycin (Gibco, Dublin, Ireland)) supplemented with 1X N2 (Gibco) and 1X B27 (Gibco), 1.25 mM N-Acetylcysteine (Sigma-Aldrich), 10% (v/v) RSPO1 conditioned media (homemade), 10 nM recombinant human (Leu15)-gastrin I (GAS, Sigma-Aldrich), 50 ng/mL EGF (Peprotech), 25 ng/mL Noggin (Peprotech), 100 ng/mL Article Title: Accelerated production of human epithelial organoids in a miniaturized spinning bioreactor. Article Snippet: .. For pancreatic ductal organoids (PDOs) culture, EM composition for PDO expansion was prepared as previously described46: Basal medium (Advanced DMEM/F12 (AD, Gibco), containing 1% (v/v) GlutaMax (Gibco), HEPES (10 mM, Gibco) and 1% (v/v) penicillin-streptomycin (Gibco, Dublin, Ireland)) supplementedwith 1XN2 (Gibco) and 1XB27 (Gibco), 1.25mM N-Acetylcysteine (SigmaAldrich), 10% (v/v) RSPO1 conditionedmedia (homemade), 10 nM recombinant human (Leu15)-gastrin I (GAS, Sigma-Aldrich), 50 ng/ mL EGF (Peprotech), 25 ng/mL Noggin (Peprotech), 100 ng/mL Article Title: Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays Article Snippet: .. Briefly, PDAC cells were obtained from the biopsies through slight digestion with the Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach , Germany) at 37 °C for 5 min. Isolated cells were placed into 12-well plates (Corning, #3513) coated with 150 μl growth factor reduced matrigel (Corning, Wiesbaden, Germany) and cultured with advanced DMEM/F12 supplemented with hydroxyethyl-piperazineethanesulfonic acid (HEPES) (10 mmol/L Thermo Fisher, Waltham, MA, USA), Wnt family member 3 A (WNT3A)-conditioned medium (30% v/v), R-spondin-1 (RSPO1)-conditioned medium (10% v/v); Article Title: Enhanced Maturity and Functionality of Vascularized Human Liver Organoids through 3D Bioprinting and Pillar Plate Culture Article Snippet: The EPCs and VPCs were mixed in the ratio of 4:1, suspended in Cultrex basement membrane extract, type 2 (BME-2; R&D Systems; 3532-010-02), and dispensed in a 24-well plate to form 50 μL BME-2 domes for culture. .. After gelation of BME-2 at 37°C for 10 - 12 minutes, the cell population was differentiated in advanced DMEM/F12 (Gibco; 12634) supplemented with 1% B27 supplement (Gibco), 1.25 mM N-acetyl-L-cysteine (Sigma; A9165), 10 nM recombinant human (Leu )-gastrin I (Sigma; G9145), 100 ng/mL Article Title: Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modelling Article Snippet: .. In brief, dissociated single cells were seeded in a collagen IV (Cat#C7521, Sigma)-precoated cell culture plate (6-well; Cat#657160, Greiner Bio-One) and cultured in isolation medium consisting of 50% (v/v) BEpiCM-b (Cat#3211, Sciencell) and 44% (v/v) advanced DMEM/F-12 (Ad-DF; Cat#12634-028, Thermo Fisher Scientific) supplemented with 2% (v/v) B-27 Supplement, serum free (Cat#17504001, Thermo Fisher Scientific), 10 mM HEPES (Cat#15630080, Thermo Fisher Scientific), 1% (v/v) GlutaMAX supplement (Cat#35050-061, Thermo Fisher Scientific), 1% (v/v) penicillin/streptomycin (Cat#15070-063, Thermo Fisher Scientific), 0,5 μg/mL Hydrocortisone (Cat#H0888, Sigma-Aldrich), 1,25 mM N-Acetyl-L-cysteine (Cat#A9165, Sigma-Aldrich), 100 μg/mL Primocin (Cat#ant-pm-2, InvivoGen), 1 μM ALK5 inhibitor A83-01 (Cat#2939/10, Tocris), 0,5 μg/mL (±)-Epinephrine hydrochloride (Cat#E4642, Sigma-Aldrich), 5 μM Y-27632 (Cat#S1049, Selleck Chemicals), 2% RSPO3-Fc Fusion Protein conditioned medium (Cat#R001 - 100 mL, U-Protein Express), 50 nM Human Heregulin-beta 1 (Cat#100-03, PeproTech), 100 ng/mL Isolation:Article Title: Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays Article Snippet: .. Briefly, PDAC cells were obtained from the biopsies through slight digestion with the Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach , Germany) at 37 °C for 5 min. Isolated cells were placed into 12-well plates (Corning, #3513) coated with 150 μl growth factor reduced matrigel (Corning, Wiesbaden, Germany) and cultured with advanced DMEM/F12 supplemented with hydroxyethyl-piperazineethanesulfonic acid (HEPES) (10 mmol/L Thermo Fisher, Waltham, MA, USA), Wnt family member 3 A (WNT3A)-conditioned medium (30% v/v), R-spondin-1 (RSPO1)-conditioned medium (10% v/v); Article Title: Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modelling Article Snippet: .. In brief, dissociated single cells were seeded in a collagen IV (Cat#C7521, Sigma)-precoated cell culture plate (6-well; Cat#657160, Greiner Bio-One) and cultured in isolation medium consisting of 50% (v/v) BEpiCM-b (Cat#3211, Sciencell) and 44% (v/v) advanced DMEM/F-12 (Ad-DF; Cat#12634-028, Thermo Fisher Scientific) supplemented with 2% (v/v) B-27 Supplement, serum free (Cat#17504001, Thermo Fisher Scientific), 10 mM HEPES (Cat#15630080, Thermo Fisher Scientific), 1% (v/v) GlutaMAX supplement (Cat#35050-061, Thermo Fisher Scientific), 1% (v/v) penicillin/streptomycin (Cat#15070-063, Thermo Fisher Scientific), 0,5 μg/mL Hydrocortisone (Cat#H0888, Sigma-Aldrich), 1,25 mM N-Acetyl-L-cysteine (Cat#A9165, Sigma-Aldrich), 100 μg/mL Primocin (Cat#ant-pm-2, InvivoGen), 1 μM ALK5 inhibitor A83-01 (Cat#2939/10, Tocris), 0,5 μg/mL (±)-Epinephrine hydrochloride (Cat#E4642, Sigma-Aldrich), 5 μM Y-27632 (Cat#S1049, Selleck Chemicals), 2% RSPO3-Fc Fusion Protein conditioned medium (Cat#R001 - 100 mL, U-Protein Express), 50 nM Human Heregulin-beta 1 (Cat#100-03, PeproTech), 100 ng/mL Cell Culture:Article Title: Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays Article Snippet: .. Briefly, PDAC cells were obtained from the biopsies through slight digestion with the Tumor Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach , Germany) at 37 °C for 5 min. Isolated cells were placed into 12-well plates (Corning, #3513) coated with 150 μl growth factor reduced matrigel (Corning, Wiesbaden, Germany) and cultured with advanced DMEM/F12 supplemented with hydroxyethyl-piperazineethanesulfonic acid (HEPES) (10 mmol/L Thermo Fisher, Waltham, MA, USA), Wnt family member 3 A (WNT3A)-conditioned medium (30% v/v), R-spondin-1 (RSPO1)-conditioned medium (10% v/v); Article Title: Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modelling Article Snippet: .. In brief, dissociated single cells were seeded in a collagen IV (Cat#C7521, Sigma)-precoated cell culture plate (6-well; Cat#657160, Greiner Bio-One) and cultured in isolation medium consisting of 50% (v/v) BEpiCM-b (Cat#3211, Sciencell) and 44% (v/v) advanced DMEM/F-12 (Ad-DF; Cat#12634-028, Thermo Fisher Scientific) supplemented with 2% (v/v) B-27 Supplement, serum free (Cat#17504001, Thermo Fisher Scientific), 10 mM HEPES (Cat#15630080, Thermo Fisher Scientific), 1% (v/v) GlutaMAX supplement (Cat#35050-061, Thermo Fisher Scientific), 1% (v/v) penicillin/streptomycin (Cat#15070-063, Thermo Fisher Scientific), 0,5 μg/mL Hydrocortisone (Cat#H0888, Sigma-Aldrich), 1,25 mM N-Acetyl-L-cysteine (Cat#A9165, Sigma-Aldrich), 100 μg/mL Primocin (Cat#ant-pm-2, InvivoGen), 1 μM ALK5 inhibitor A83-01 (Cat#2939/10, Tocris), 0,5 μg/mL (±)-Epinephrine hydrochloride (Cat#E4642, Sigma-Aldrich), 5 μM Y-27632 (Cat#S1049, Selleck Chemicals), 2% RSPO3-Fc Fusion Protein conditioned medium (Cat#R001 - 100 mL, U-Protein Express), 50 nM Human Heregulin-beta 1 (Cat#100-03, PeproTech), 100 ng/mL |